A pilot scale two-stage anaerobic digester treating food waste leachate (FWL): Performance and microbial structure analysis using pyrosequencing |
| |
| Institution: | 1. Center for Bioanalysis, Korea Research Institute of Standards and Science, 267 Gajeong-Ro, Yuseong-Gu, Daejeon 305-340, Republic of Korea;2. Clean Energy Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-Dong, Sungbuk-Ku, Seoul 136-791, Republic of Korea;3. Environmental R&D center, Hansol EME Co. Ltd., 7th Fl., First Tower, 266-1 Seohyeon-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-824, Republic of Korea;4. Department of Chemical Engineering, Hanyang University, 17 Hangdang-Dong, Seongdong-Ku, Seoul 133-791, Republic of Korea;1. Department of Environmental Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea;2. Department of Civil Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea;1. Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria;2. Hochschule Hamm-Lippstadt, Biotechnologie, Marker Allee 76-78, 59063 Hamm, Germany;3. Müller Abfallprojekte GmbH, Hauptstraße 34, 4675 Weibern, Austria;1. Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region;2. CSIRO Land and Water, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Underwood Avenue, Floreat, WA 6014, Australia;1. School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea;2. Infrastructure and Environment Division, School of Engineering, University of Glasgow, Glasgow G12 8LT, UK;3. Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea;1. Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium;2. Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngårdsholmsvej 49, 9000 Aalborg, Denmark;3. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. Department of Energy Engineering, Future Convergence Technology Research Institute, Gyeongsang National University, 501 Jinju-daero, Jinju, Gyeongnam 52828, Republic of Korea;2. Department of Environmental Engineering, College of Engineering, Korea Maritime and Ocean University, 727 Taejong-ro, Yeongdo-gu, Busan 49112, Republic of Korea;3. Division of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Republic of Korea |
| |
| Abstract: | Food waste leachate (FWL) from the food waste recycling facilities in Korea is a serious environmental problem. Much research was done on anaerobic digestion of FWL in a lab-scale; however, there is little information on a large scale anaerobic digestion system (ADS). In this study, a two-phase ADS in a pilot scale was operated using FWL and the ADS performance and microbial structure dynamics using pyrosequencing were investigated. The ADS was operated for 136 days using FWL containing a high concentration of volatile fatty acid (12,435 ± 2203 mg/L), exhibiting volatile acid (VS) removal efficiency of 74–89% and CH4 yield of 0.39–0.85 Nm3/kg of reduced VS. The microbial structure at 76, 101, and 132 days indicated the methanogen population shift from acetoclastic methanogens (Methanosarcina and Methanosaeta) to hydrogenotrophic methanogens (Methanobacterium and Methanoculleus). The bacterial community also shifted to the taxa syntrophically related with hydrogenotrophic methanogens (Clostridia). The statistical analysis revealed the positive correlation of VS removal efficiency with Methanosarcina, but the negative correlation with Methanobacterium. The results presented here suggest that acetoclastic methanogens and their associated bacteria were more efficient for VS removal in the pilot scale ADS system, providing useful information for FWL treatment in a large scale ADS. |
| |
| Keywords: | Food waste leachate Anaerobic digestion Pilot-scale Pyrosequencing Methanogen |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
