| 亚天花粉蛋白突变体(R163H n-TCS和R163Q n-TCS)的晶体结构研究 |
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| 引用本文： | 李会广,吴伸,颜丽,李建辉,黄岳顺,施庆利,董贻诚. 亚天花粉蛋白突变体(R163H n-TCS和R163Q n-TCS)的晶体结构研究[J]. 生物物理学报, 1999, 15(2): 277-282 |
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| 作者姓名： | 李会广 吴伸 颜丽 李建辉 黄岳顺 施庆利 董贻诚 |
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| 作者单位： | 中国科学院生物物理所，香港浸会大学生物系 |
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| 基金项目： | 国家自然科学基金,香港研究基金委员会基金,香港浸会大学院务研究基金 |
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| 摘 要： | 用悬滴汽相扩散法得到了Ｒ１６３Ｈｎ－ＴＣＳ和Ｒ６１３Ｑｎ－ＴＣＳ的晶体，Ｍａｒ－Ｒｅｓｅａｒｃｈ面探测器系统上分别收集了０．２００和０．２０５ｎｍ分辨率的Ｘ－射线衍射数据，采用同晶差值傅立叶法解析结构，用Ｘ－ＰＬＯＲ软件包进行修正，最后的晶体学Ｒ因子分别为０．１８４和０．１８５，键长偏差分别为０．００１３ｎｍ和０．００１４ｎｍ，键角偏差分别为２．５９０和２．８１５，结构测定显示Ｒ１６３Ｈｎ－ＴＣＳ
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| 关 键 词： | 突变体 晶体结构 亚天花粉蛋白 |
CRYSTAL STRUCTURE OF R163H n-TCS and R163Q n-TCS |
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| Li Huiguang Wu Shen Yan Li Li JianhuiWong Ricky N.S Shi Qingli Dong Yicheng. CRYSTAL STRUCTURE OF R163H n-TCS and R163Q n-TCS[J]. Acta Biophysica Sinica, 1999, 15(2): 277-282 |
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| Authors: | Li Huiguang Wu Shen Yan Li Li JianhuiWong Ricky N.S Shi Qingli Dong Yicheng |
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| Affiliation: | Li Huiguang Wu Shen Yan Li Li JianhuiWong Ricky N.S* Shi Qingli* Dong Yicheng |
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| Abstract: | Crystals of R163H n-TCS and R163Q n-TCS were grown by hanging drop vapor diffusion technique. X-ray diffraction data of two crystals were collected respectively to 0.200 and 0.205nm,on Mar-Research IP. The Structure were determined by Difference Fourier Method, the rms deviations of bond length were 0.0013nm and 0.0014nm,and r.m.s deviations of bond angle are 2.590° and 2.825° respectively. Structure determination indicates that there is no obvious change between R163H n-TCS, R163Q n-TCS and the original n-TCS, the structures of the active pocket remain unchanged. Biochemical analysis shows that suggests R163H n-TCS has the glycosidase activity while R163Q n-TCS has no. All findings suggest that the R163 is one of the critical residuels for N-glycosidase activity of n-TCS. |
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| Keywords: | n-TCS Mutants Crystal Structure |
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