Distribution of Coliphages Against Four <Emphasis Type="Italic">E. Coli</Emphasis> Virotypes in Hospital Originated Sewage Sample and a Sewage Treatment Plant in Bangladesh |
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Authors: | Muntasir Alam Tasmia Farzana Chowdhury Rafiqul Ahsan Mahmuda Yasmin Jamalun Nessa |
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Institution: | (1) Department of Microbiology, University of Dhaka, Dhaka, 1000, Bangladesh |
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Abstract: | The distribution of coliphages infecting different Escherichia coli virotypes (EHEC, EIEC, EPEC, ETEC) and an avirulent strain (K-12) in sewage system of a hospital and a sewage treatment plant
(STP) was investigated by culture-based agar overlay methods. Coliphages were found in all the samples except stool dumping
site in the sewage system of the hospital and lagoon of the STP. Bacteriophage count (pfu/ml) infecting E. coli strains showed the following ascending pattern (EHEC < EIEC < EPEC < ETEC < E coli K-12) in all the collected samples except one. Phages capable of infecting avirulent E. coli K-12 strains were present in the highest number among all the examined locations. Phages specific for E. coli K-12 presented high diversity in plaque size on the bacterial lawn. Virulent E. coli specific coliphages rarely produced plaques with diameter of 1–2 mm or over. Conventional agar overlay method was found to
be not satisfactory for phage community analysis from primary stool dumping site of the hospital, probably due to the presence
of high concentration of antimicrobial substances. The gradual decrease seen in the five groups of coliphage quantity with
the ongoing treatment process and then the absolute absence of coliphages in the outlet of the examined treatment plant is
indicative of the usefulness of the treatment processes practiced there. |
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Keywords: | E coli Virotypes Bacteriophages Sewage treatment plant Hospital sewage |
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