Responses of <Emphasis Type="Italic">Rhodotorula</Emphasis> sp. Y11 to cadmium |
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Authors: | ZhiJian Li HongLi Yuan |
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Institution: | (1) College of Biological Sciences, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, China Agricultural University, Beijing, 100094, People’s Republic of China |
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Abstract: | Some aspects of the cellular responses to cadmium were extensively investigated in the yeast Rhodotorula sp. Y11. Scanning electron microscopy indicated that accumulation of cadmium in the Y11 did not cause any visible effects
on cell morphology. More than 20% yeast cells still showed viability after 15 h of cadmium accumulation under 100 mg l−1 cadmium concentration, and transmission electron microscopy analysis showed that plasmolysis and thickened cell wall were
not observed in all of the cells. In the presence of cadmium, the activities of superoxide dismutase (SOD) and catalase (CAT)
were all greater than the control, but the increase was in a dose-independent manner. Changes in SOD and CAT activities were
also dependent on the time of exposure. Therefore, it suggests that antioxidative defenses play an important role in cadmium
tolerance in Rhodotorula sp. Y11. Nondenaturing polyacrylamide gels revealed only one SOD isoforms in Y11 even under exposure to cadmium. |
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Keywords: | Cadmium Catalase Rhodotorula sp Resistance Superoxide dismutase |
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