Protein engineering by cell-surface display |
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| Authors: | Wittrup K D |
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| Affiliation: | Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. wittrup@mit.edu |
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| Abstract: | Protein libraries displayed on cell surfaces can be labeled with soluble ligands exhibiting well-characterized binding equilibria and dissociation kinetics, and then quantitatively screened by flow cytometry at a rate of >10(4) clones/second. The promise of cell-surface display for directed evolution is being realized, with significant improvements recently reported in protein ligand binding affinity, stability, expression and enzymatic activity. |
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