Identification of a DNA methylation point in the promoter region of the bovine CYP21 gene |
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| Authors: | da Silva A M de Freitas M A R Rios A F L Renzi A Lôbo R B Galerani M A V Vila R A Ramos E S |
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| Institution: | Departamento de Genética, Faculdade de Medicina de Ribeir?o Preto, Universidade de S?o Paulo, Ribeir?o Preto, SP, Brasil. |
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| Abstract: | The CYP21 (steroid 21-hydroxylase) gene is involved in the synthesis of steroid hormones. Bov-A2 is a retroposon that is common in ruminant genomes. The promoter region of bovine CYP21 contains a short interspersed nucleotide element of Bov-A2, which overlaps a putative Sp1 binding site. We looked for RFLP/HpaII polymorphism in the Bov-A2 element in bovine Zebu breeds by PCR-RFLP, and examined whether polymorphism in this element is associated with methylation. Among DNA samples from 135 Brazilian Zebu breed cattle, we identified an RFLP/HpaII polymorphism (T/C), which, based on a restriction methylation-sensitive assay employing HpaII and isoschizomer MspI enzymes (methylation-sensitive and -non-sensitive enzymes, respectively), appears to be a DNA methylation point. This is the first report of this polymorphism and on DNA methylation in the bovine CYP21 promoter region in Brazilian Zebu cattle. |
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