Carbonic Anhydrase of Chlamydomonas: Purification and Studies on its Induction Using Antiserum against Chlamydomonas Carbonic Anhydrase |
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Authors: | Yang, Shi-Yuan Tsuzuki, Mikio Miyachi, Shigetoh |
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Affiliation: | Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan |
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Abstract: | Carbonic anhydrase (EC 4.2.1.1[EC]; CA) was purified by affinitychromatography from cells of the unicellular green alga Chlamydomonasreinhardtii which had been grown photoautotrophically in ordinaryair. Antiserum raised in rabbit against this purified CA crossreactedwith Chlamydomonas CA but not with spinach leaf CA nor bovineerythrocyte CA. When the CO2 concentration provided to the algalcells was decreased from 4% to the ordinary air level (0.04%),CA activity and the content of CA protein determined by theimmunodiffusion test showed parallel increases. In contrast,when the CO2 concentration was raised from air level to 4% CO2CA activity and its content expressed on the basis of culturevolume remained rather constant. These results indicate thatsynthesis of the CA protein is induced when the CO2 concentrationis lowered from 4 to 0.04% during algal growth. On the otherhand, the synthesis of CA stops when CO2 concentration is raisedfrom air level to 4%. (Received June 30, 1984; Accepted October 8, 1984) |
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