过表达miR-455抑制宫颈癌细胞SiHa的生长

研究表明，microRNA(miRNA)可作为癌基因或抑癌基因发挥功能、调控细胞增殖和凋亡等生物学行为，与肿瘤的发生发展密切相关. 在本研究中，我们检测了miR- 455在宫颈癌组织中的表达变化及其对宫颈癌SiHa细胞生物学功能的影响. Real- time PCR实验结果显示,miR-455在宫颈癌组织样本中较正常宫颈组织表达明显降低. 瞬时转染miR-455 mimics使其在SiHa细胞中过表达. CCK-8及流式细胞术分析显示, 过表达miR-455明显抑制细胞增殖，促进细胞凋亡，导致细胞G1/S期阻滞. Real- time PCR分析显示,PI3KR1，BCL2L2 mRNA明显降低.上述研究结果表明， miR-455可显著降低SiHa细胞存活能力，是一个潜在的抑癌基因.

Overexpression of miR-455 Inhibits Proliferation in SiHa Cells

Recent studies have demonstrated that microRNAs might function as oncogenes or tumor suppressors. They can regulate cell proliferation and apoptosis that are important in tumor formation and development. In this study, we detected the expression levels of miR-455 in human cervical cancer samples and investigated the effects of miR-455 on the biological behavior of cervical cancer SiHa cell line. Real-time PCR analysis exhibited that the levels of miR-455 was markedly downregulated in cervical cancer samples compared with normal cervical samples. In the CCK8 and flow cytometry assay, ectopic expression of the miR-455 promotes cell apoptosis, inhibits proliferation and arrests cell cycle. In addition, real-time PCR analysis exhibited that overexpression of miR-455 decrease PI3KR1 and BCL2L2 mRNA. These findings suggested that miR-455 might function as a tumor suppressor to inhibit cell viability in cervical cancer cells.