In vitro binding of nucleolin to double-stranded telomeric DNA |
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Authors: | Pollice A Zibella M P Bilaud T Laroche T Pulitzer J F Gilson E |
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Affiliation: | Dipartimento di Genetica, Biologia Generale e Molecolare, Università degli Studi di Napoli Federico II, via Mezzocannone 8, Naples, 80134, Italy. pollice@biol.dgbm.unina.it |
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Abstract: | We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucleoli in growing Hela cells. We demonstrate that a truncated form of human nucleolin and a purified deletion derivative of mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggests a new link between telomeres and the nucleolus. |
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