| 灰绿拟青霉U—2发酵液杀蚜活性物质的分离提取 |
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| 引用本文： | 戴美学 祖爱民. 灰绿拟青霉U—2发酵液杀蚜活性物质的分离提取[J]. 生物技术, 1997, 7(3): 32-34,36 |
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| 作者姓名： | 戴美学 祖爱民 |
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| 作者单位： | [1]山东师范大学生物系 [2]济南教育学院生物系 |
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| 摘 要： | 采用活性炭吸附、甲醇洗脱、高效液相色谱分离方法从灰绿拟青霉Ｕ－２发酵液中提取获得一种脂溶性杀蚜性组分，暂名为组分Ａ，为淡黄色棒杆状结晶。通过乙酸乙脂反复萃取证明发酵液中水溶性杀蚜活性成分存在，但暂未获得纯品。
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| 关 键 词： | 灰绿拟青霉 杀蚜活性物 分离 |
The Seperation and Extraction of Aphis-Killing Substance in Paecilomyces Griseoviride U - 2 Strain Fermentation Liquid |
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| Dai Meixue. The Seperation and Extraction of Aphis-Killing Substance in Paecilomyces Griseoviride U - 2 Strain Fermentation Liquid[J]. Biotechnology, 1997, 7(3): 32-34,36 |
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| Authors: | Dai Meixue |
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| Abstract: | A fat soluble aphis-killing substance, named component A for time being, which is yellowish rod-shaped crystal, was separated and purified out from the fermentation liquid of Paecilomyces griseoviride U-2 strain by using methods of activated carbon adsorption,methyl alcohol washing and high performance liquid chromatography.It is testified that there is water soluble aphis-killing component in fermentation liquid by repeated extraction with acetic ester, but so far the water soluble component has not been purified. |
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| Keywords: | Paecilomyces griseovirideaphis-killing component high performance liquid chromatography(HPLC) |
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