| 产鸟苷的枯草杆菌缺失GMP还原酶活性突变株的选育 |
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| 引用本文： | 柏建新,邓崇亮.产鸟苷的枯草杆菌缺失GMP还原酶活性突变株的选育[J].生物技术,1997,7(3):25-28,31. |
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| 作者姓名： | 柏建新 邓崇亮 |
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| 作者单位： | 江苏省微生物研究所!无锡 |
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| 摘 要： | 以枯草杆菌ＳＭ－１２－２为出发菌株，经物理化学诱变剂连续处理，获得一株８－氮杂鸟嘌呤（８－ＡＧ），缺失鸟苷酸（ＧＭＰ）还原酶性的突变株Ｇ－２０５。该突变株肌苷酸（ＩＭＰ）脱氢酶活性比亲株高，在培养基中积累５．１７ｍｇ／ｍｌ鸟苷，９．８４ｍｇ／ｍｌ肌苷。
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| 关 键 词： | 工业微生物 枯草杆菌 鸟苷 突变株 鸟苷酸还原酶 |
Breeding of Bacillus subtilis G-205 with Lack-GMP Reductase for Production of Guanosine |
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| Bai Jianxin ,Deng Chongliang.Breeding of Bacillus subtilis G-205 with Lack-GMP Reductase for Production of Guanosine[J].Biotechnology,1997,7(3):25-28,31. |
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| Authors: | Bai Jianxin Deng Chongliang |
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| Abstract: | A inosine producing strain of Bacillus subtilis SM-12-2 were mutated by UV-ray,EMS and NTG repeatedly, then a strain of Bacillus subtilis G-205 with 8-azaguanine resis-tant and lack-guanylic acid (GMP ) reductase activity were obtained.The inosiric acid(IMP) debydrogenase activity in the strain of B. subtilis G-205 was highter than that ofparent strain. The amount of guanosine and inosine which were accumunted by this mutantin the fermention culture was 5. 17mg/ml and9.84mg/nl respectively. |
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| Keywords: | Inosine Guanosine Lack-GMP reductase 8-Azaguanine resistant |
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