| 酶法合成中丙氨酸的检测 |
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| 引用本文： | 胡永红 蔡兴怀. 酶法合成中丙氨酸的检测[J]. 生物技术, 1997, 7(1): 20-22 |
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| 作者姓名： | 胡永红 蔡兴怀 |
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| 作者单位： | 南京化工大学生物工程与科学系!210009 |
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| 摘 要： | 本文建立了丙氨酸在醋酸缓冲液中形成丙氨酸-铜配离子及其十二烷基磺酸配离子对，使其紫外无吸收的丙氨酸在230nm处于有强紫外吸收，从而对酶法合成中的丙氨酸进行定量分析．该法在0～50mg／L范围内有良好的线性关系，直线方程为A(230)=0.01712·C（n=5）C：mg／L）。相关系数为0．9994，回收率为96．8％～104．0％，且该法不受酶反应液的影响，实验结果证明该检测体系简单、实用、测定结果可靠。
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| 关 键 词： | 丙氨酸-铜配合物 紫外吸收 检测 |
Determination of Alanine in Enzymatic Synthesis |
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| Hu Yonghong, Cai Xinghuai ,Yang WengeChen Wuling ,Ouyang Pingkai. Determination of Alanine in Enzymatic Synthesis[J]. Biotechnology, 1997, 7(1): 20-22 |
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| Authors: | Hu Yonghong Cai Xinghuai Yang WengeChen Wuling Ouyang Pingkai |
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| Abstract: | A new method for determination of alanine with maxium absorptivity ar 230nm by formation of alanine-copper ion pairs with sodium dodecylsulfate was estabolished' When the concentration was about O~ 5omg/L, the calibration curve was A230=0. 01712C (n= 5, C:mg/l) and the relation coefficient, the recovery were 0. 9994, 96. 8%~ 104. 0% respectivelyIt could effectively avoid the interfereme of medium and the system is simple, practial and reliable. |
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| Keywords: | Alanine-copper pair UV absorption Determination |
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