Abstracts of papers read at the Winter Meeting, 1961 |
| |
Authors: | Aika Yamaguchi Mona Hoppenrath Vera Pospelova Takeo Horiguchi Brian S Leander |
| |
Institution: | 1. Departments of Botany and Zoology , University of British Columbia, #3529?-?6270 University Boulevard , Vancouver, BC, V6T 1Z4, Canada aikay@interchange.ubc.ca;3. Departments of Botany and Zoology , University of British Columbia, #3529?-?6270 University Boulevard , Vancouver, BC, V6T 1Z4, Canada;4. Senckenberg Research Institute, German Centre for Marine Biodiversity Research , Südstrand 44, D-26382 Wilhelmshaven, Germany;5. School of Earth and Ocean Sciences, University of Victoria, OEASB A 405 , P.O. Box 3065 STN CSC, Victoria, BC, V8W 3V6, Canada;6. Department of Natural History Sciences, Faculty of Science , Hokkaido University, North 10 , West 8, Kita-ku, Sapporo 060-0810, Japan;7. Departments of Botany and Zoology , University of British Columbia, #3529?-?6270 University Boulevard , Vancouver, BC, V6T 1Z4, Canada |
| |
Abstract: | Herdmania litoralis is a heterotrophic, sand-dwelling dinoflagellate with morphological characters that do not provide clear evidence for its systematic position in any existing family of dinoflagellates. Protoperidinium minutum is a heterotrophic, planktonic species that has a typical tabulation for the genus Protoperidinium. In order to infer the phylogenetic positions of these two species more confidently, we characterized the thecal plate patterns and determined small-subunit and large-subunit ribosomal DNA sequences (SSU rDNA and LSU rDNA, respectively) from both species. Intraindividual and intraspecific diversity of SSU and LSU rDNA data were characterized in H. litoralis using a combination of single-cell PCR approaches and analyses of PCR clones derived from multi-cell DNA extractions. The results of the molecular phylogenetic analyses demonstrated a novel, well-supported clade comprising both sand-dwelling species (H. litoralis and Thecadinium dragescoi) and planktonic species (P. minutum). Because the establishment of this clade also demonstrated that P. minutum is not a member of Protoperidinium, we reinstated and emended the genus Archaeperidinium Jörgensen 1912 Jörgensen, E. 1912. Bericht über die von der schwedischen hydrographisch-biologischen Kommission in den schwedischen Gewässern in den Jahren 1909–10 eingesammelten Planktonproben. Svenska Hydrograph.-Biol. Komm. Skr., 4: 1–20. Google Scholar]. |
| |
Keywords: | Archaeperidinium Herdmania litoralis heterotrophic dinoflagellates intraspecific variability LSU rDNA Protoperidiniaceae Protoperidinium minutum single-cell PCR SSU rDNA Thecadinium dragescoi |
|
|