A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis |
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Authors: | Mueller C Hutter P Sahli R Bretz A Toutoungi L N Péter O |
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Affiliation: | Infectious Disease and Immunology, Institut Central des H?pitaux Valaisans, Sion, Switzerland. |
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Abstract: | Borrelia garinii is one of the three major Borreliae responsible for Lyme borreliosis in Europe. We have characterized a protein of B. garinii (VS102) and a genomic fragment from the gene encoding this protein was cloned. The DNA sequence of the fragment showed high homology with a known gene of B. burgdorferi sensu stricto. The protein encoded by this gene in B. burgdorferi sensu stricto is a phosphocarrier protein (histidine-containing protein). A mutation T to G polymorphism at codon 57 was found to be specific to B. garinii. A PCR-based approach that allows the rapid detection of this mutation made it possible to specifically discriminate B. garinii from other B. burgdorferi genospecies with high sensitivity and specificity. |
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Keywords: | Borrelia garinii Lyme borreliosis Mutagenic polymerase chain reaction Phosphocarrier protein HPR |
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