Effects of serum deprivation on myofibrillar proteolysis in chick myotube cultures |
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Authors: | Nakashima Kazuki Nonaka Itoko Masaki Shigehiko |
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Institution: | Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan. kaznaka@affrc.go.jp |
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Abstract: | We examined the effects of serum deprivation on myofibrillar proteolysis in chick myotubes. Myotubes were incubated with serum-free medium for 24 hours. N(tau)-methylhistidine release, as an index of myofibrillar proteolysis, as well as protease activities such as calpain, proteasome, and cathepsins (B+L and D) activities were increased by serum deprivation. These results indicate that serum deprivation induces calpain, proteasome, and cathepsins activities, resulting in an increase in myofibrillar proteolysis in chick myotubes. |
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