Effect of mitomycin C and bromodeoxyuridine on Fanconi anemia lymphocytes. |
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| Authors: | S Frias S Mendoza B Molina A Carnevale |
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| Affiliation: | Departamento de Genetica Humana, Instituto Nacional de Pediatría, Mexico D.F. |
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| Abstract: | The authors studied the effect of mitomycin C (MMC) and bromodeoxyuridine (BrdU) on the induction of chromosome aberrations on lymphocytes of four patients with Fanconi anemia (FA) and of one normal subject. A control culture and six experiments were designed to test the possible synergic effect of MMC and BrdU. Their results revealed no evidence of MMC-BrdU synergism on the induction of chromosome aberrations in FA lymphocytes. However, chromosomes showed more damage when FA cells were harvested 24 h after MMC stress than when cells were harvested shortly after treatment. This can be explained by a DNA repair defect or by a toxic effect of oxygenation of cells during the procedure. |
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