The ilvEDA operon of Escherichia coli K12 encodes only one valine-alpha-ketoglutarate transaminase activity. |
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Authors: | C W Adams R P Lawther G W Hatfield |
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Institution: | Department of Microbiology College of Medicine University of California, Irvine Irvine, California 92717 USA |
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Abstract: | Transaminase B of K12 was purified to apparent homogeneity as measured by SDS acrylamide gel electrophoresis, immunoelectrophoresis, and amino terminal sequence analysis. The valine- and isoleucine-α-ketoglutarate dependent transaminase activities of pure enzyme as well as crude extracts were characterized by immunologic and kinetic methods. The data disprove the existence of a separate valine-α-ketoglutarate transaminase within the operon. |
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