In vitro induction of tetraploidy in mulberry (Morus alba L.) |
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Authors: | S P Chakraborti K Vijayan B N Roy S M H Qadri |
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Institution: | (1) Central Sericultural Research and Training Institute, Berhampore-742 101, West Bengal, India, IN |
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Abstract: | A high frequency of tetraploidy was induced in mulberry (Morus alba L.) through apical bud treatment under in vitro conditions. Apical buds from in vitro-grown plants were treated with three different concentrations (0.05, 0.1 and 0.2%)
of colchicine in MS medium for 24 h. Tetraploidy at a frequency of 39.4±4.8% was obtained using 0.1% colchicine, whereas the
frequency of tetraploidy was significantly reduced to 16.7±2.3% when 0.2% colchicine was used. Morphological, histological
and cytological evidence indicated a phenotypic and genomic similarity of in vitro- with ex vitro-induced tetraploids. Rooting
of tetraploids was on basal medium containing 2.6 μm NAA. The recovery of tetraploids was 80.8% more efficient using the in vitro method instead of the ex vitro method. The use
of the same colchicine medium for up to 4 weeks with additional explants was found to be equally effective for the induction
of tetraploidy.
Received: 6 January 1997 / Revision received: 6 October 1997 / Accepted: 12 December 1997 |
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Keywords: | Apical buds Colchicine In vitro induction Tetraploids Mulberry |
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