Isolation and characterization of a temperature-sensitive conditional mutant of Escherichia coli altered for the control of phosphate-regulated proteins |
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Authors: | Jean-Louis Uribellarea Martine Heyde Raymond Portalier |
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Institution: | Centre de Recherche de Biochimie et de Génétique Cellulaire du CNRS, Toulouse, France. |
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Abstract: | We have identified a conditional mutation which confers a ple?otropic phenotype to Escherichia coli cells: no growth at temperature higher than 36 degrees C, an altered control of the synthesis of several phosphate-regulated polypeptides (including alkaline phosphatase, sn-glycerol-3-phosphate binding protein, phosphate binding protein and outer membrane porin protein PhoE) after growth at 36 degrees C and a wild-type phenotype at 30 degrees C. This mutation was located at minute 89.5 on the E. coli chromosome in a gene we have called cpr for conditional phosphate-regulated. |
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Keywords: | Escherichia coli Conditional mutant Phosphate-regulated protein |
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