Partial purification of beta-N-acetylhexosaminidase A by affinity chromatography

A glycopeptide derived from bovine nasal septum by sequential treatment with trypsin, chymotrypsin, 0.05N HCl in dry methanol (desulfation), testicular hyaluronidase and β-glucuronidase, was coupled to Sepharose-4B in the presence of cyanogen bromide. β-$\text{N}$-Acetylhexosaminidase A was selectively retarded when crude extracts of human skin fibroblasts or liver were applied to the affinity column and was subsequently eluted with 0.1% Triton X-100 in 0.1M citrate-phosphate buffer pH 4.4, providing a simple method for purification.