Cloning and characterization of VIGG,a novel virus-induced grapevine protein,correlated with fruit quality |
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| Authors: | Hironori Katoh Shunji Suzuki Toshiyuki Saitoh Tsutomu Takayanagi |
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| Institution: | 1. Scientific Research and Experiment Center, The Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710004, PR China;2. The Key Laboratory of Environment and Gene Related to Diseases, Xi’an Jiaotong University, Ministry of Education, Xi’an 710061, PR China;1. Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China;2. Guangzhou Institute for Criminal Science and Technology & Guangzhou Department of Police, Guangzhou 510030, China;1. Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China;2. Guangzhou Institute for Criminal Science and Technology & Guangzhou Department of Police, Guangzhou 510030, China;1. Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China;2. Department of Neurology, First Affiliated Hospital of Inner Mongolia Medical College, Hohhot, China;1. Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Paraje “El Pozo”, Santa Fe, CP: 3000, Argentina;2. Servicio de Clínica Médica, Hospital J.B “Iturraspe”, Bv. Pellegrini 3551, Santa Fe, CP: 3000, Argentina;3. Instituto de Inmunología, Facultad de Medicina, Universidad Nacional de Rosario, Santa Fe 3102, Rosario, CP: 2002, Santa Fe, Argentina |
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| Abstract: | We report here the identification and characterization of VIGG, a novel virus-induced grapevine protein. Analysis of VIGG expression in grapevine demonstrated that VIGG was constitutively expressed in leaves and stems in virus-infected grapevine, and that VIGG expression was induced by grapevine virus A (GVA) infection, but not by infection with other viruses. The virus-induced expression profile of VIGG was supported by the finding that virus-free meristem cultures prepared from virus-infected grapevines did not express VIGG. An experiment using GFP–VIGG fusion protein demonstrated that VIGG might be localized in or around the endoplasmic reticulum (ER). Treatment of grapevine cells with ER stress inducers resulted in the induction of VIGG expression. Berries from VIGG-expressing grapevines had higher organic acid and phenolic contents than those from control grapevines that did not express VIGG. Interestingly, fruit composition of a grapevine that was simultaneously infected by GVA and grapevine virus B (GVB), which did not express VIGG, was significantly different from that of GVA-infected grapevines expressing VIGG, suggesting that the effector of fruit composition alteration might be VIGG expression, but not GVA infection. Taken together, VIGG expression might suppress the decrease in organic acid content and increase phenol content in berries. Further investigation of the biological function of VIGG is expected to provide new information on the fruit quality of grapevines. |
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