Stability of DNA/Td3717 complexes for gene transfection,defined by the size and polydispersity of the complex |
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| Authors: | Shinichi Kuriyama Yasushi Taguchi Kazuto Watanabe Kanako Nishimura Kazutoshi Yanagibashi Yoshiki Katayama Takuro Niidome |
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| Institution: | 1. Pharmaceutical Research Center, Mochida Pharmaceutical Co. Ltd, Gotemba 412-8524, Japan;2. Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan;3. Center for Future Chemistry, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan;4. PRESTO, Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan |
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| Abstract: | The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing. |
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