佐剂诱导关节炎大鼠模型的建立及成纤维样滑膜细胞的分离

目的建立类风湿性关节炎（RA）动物模型;从炎性关节滑膜中分离成纤维样滑膜细胞（FLS）。方法应用热灭活结核杆菌H37Ra菌株与矿物油混合制备改良的佐剂,尾根部皮内注射Lewis大鼠诱导关节炎;剪取成功诱导关节炎大鼠的病变踝关节,从中剥离滑膜组织,充分剪碎后采用胶原酶消化法分离成纤维样滑膜细胞。结果成功诱导了大鼠关节炎,发病率为100%,发病时间有规律,组织学表现与RA相似;成功在体外培养了FLS并对其进行了鉴定,掌握了其生长形态和特征。结论成功制备RA动物模型并获得FLS,为今后RA发病机制探索和药物评价提供了良好的体内动物模型和体外细胞模型。

Establishment of a rat model of adjuvant-induced arthritis and isolation of fibroblast-like synoviocytes

Objective To establish an animal model of rheumatoid arthritis （RA） and isolate fibroblast-like sy- noviocytes （FLS） from inflammatory synovium. Methods Modified adjuvant was prepared using heat-killed Mycobacteri- um tuberculosis H37Ra with sterile mineral oil, and arthritis was induced in Lewis rats by injection of the adjuvant at base of tail. Synovial tissue specimens were taken from arthritic ankle joints followed by mincing adequately. FLS were isolated by collagenase digestion. Results Arthritis was successfully induced in the rats. The incidence reached 100% with regular time of arthritis development, and the histological changls were similar to those of rheumatoid arthritis. FLS were success- fully obtained,identified and characterized in vitro. Conclusions An animal model of RA has been successfully estab- lished in rats and FLS have been successfully obtained, providing a desirable in vivo animal model and in vitro cell model for exploration of RA pathogenesis and drug evaluation.