The ultrastructural ionic fixation technique localizing acetylcholinesterase activity,reveals simultaneously acetylcholine-like cation in the synaptic vesicles of the motor nerve terminals |
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Authors: | S. Tsuji |
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Affiliation: | (1) Laboratoire de Cytologie, Université P. et M. Curie, F-75230 Paris Cedex 05, France |
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Abstract: | Summary A new cytochemical technique is proposed for side by side localization of acetylcholine and of acetylcholinesterase activity of motor end-plate at ultrastructural level. The technique is based on the simultaneous ionic fixation of vesicular acetylcholine and of histochemical copper thiocholine precipitate with phosphomolybdic acid: the molybdic heteropolyanion forms insoluble salts with these two quaternary ammonium cations, providing in situ acetylcholine phosphomolybdate and copper thiocholine phosphomolybdate. Both of them are osmium resistant; the electron dense precipitates allow for a fine localization of acetylcholine and acetylcholinesterase activity at electron microscopic level. |
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