Interaction of Hyaluronectin with Hyaluronic Acid Oligosaccharides |
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Authors: | Philippe Bertrand Bertrand Delpech |
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Institution: | Laboratoire d'Immunochimie, Centre Henri Becquerel, Rouen, France |
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Abstract: | Hyaluronic acid was digested by bovine testicular hyaluronidase, and oligomers were fractionated by gel permeation using AcA 202 Ultrogel, an acrylamide-agarose matrix. Oligosaccharides composed of from two to six disaccharide repeating units were isolated. Two nonasaccharides were prepared by enzymatic or chemical modification of the decasaccharide. Oligosaccharides were compared by a competitive inhibition in the enzyme-linked immunosorbent assay for their ability to inhibit the interaction of hyaluronectin (a hyaluronic acid-binding brain glycoprotein) with hyaluronic acid. Among these oligosaccharides, decasaccharides were the smallest fragments that strongly inhibited the interaction. Octasaccharides inhibited with 700-fold lower affinity than decasaccharides. Dodecasaccharides had the same effect as decasaccharides. Nonasaccharides obtained by beta-glucuronidase splitting of decasaccharides inhibited the interaction more than nonasaccharides prepared by an alkaline treatment. |
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Keywords: | Enzyme-linked immunosorbent assay Hyaluronectin Hyaluronic acid Interaction Oligosaccharides |
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