Detection of immunomagnetically captured Escherichia coli O157:H7 by antibody-conjugated alkaline phosphatase |
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Authors: | Tu S I Patterson D Briggs C Irwin P Yu L |
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Institution: | (1) United States Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA, US |
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Abstract: | A rapid and sensitive detection process for Escherichia coli O157:H7 was developed using alkaline phosphatase (APase)-labeled anti-E. coli O157 antibodies to tag the targeted bacteria. Immunomagnetic beads or antibody-labeled streptavidin-coated magnetic beads
were then used to capture the APase-tagged E. coli. Immunomagnetically captured bacteria were washed and distributed into microplates or optical cuvettes. The enzyme-catalyzed
hydrolysis of p-nitro-phenol phosphate in alkaline solutions was then followed. Less than 1000 cfu/ml of E. coli O157:H7 could be detected. This approach was applied to detect the bacteria artificially spiked in beef hamburgers. Less
than 1 cfu/g of E. coli O157:H7 produced a significant response after cultural enrichment for 4–6 h at 37°C. Journal of Industrial Microbiology & Biotechnology (2001) 26, 345–349.
Received 30 August 2000/ Accepted in revised form 17 April 2001 |
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Keywords: | : immunomagnetic capture bacterial detection antibody-conjugated alkaline phosphatase bacterial detection method |
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