Versatile shuttle cloning vectors for the unicellular cyanobacterium Anacystis nidulans R2 |
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| Authors: | Reginald H. Lau Neil A. Straus |
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| Affiliation: | Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6;Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 1A1 |
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| Abstract: | Abstract 3 new shuttle cloning vectors for gene transfer into Escherichia coli and Anacystis nidulans have been constructed by utilizing the cyanobacterial origin of replication of the small plasmid pANS from A. nidulans . 2 of these new vectors, pXB7 (pDPL13 derivative) and pECAN8 (pUC8 derivative), convey ampicillin resistance, and transform A. nidulans with relatively high frequencies. Vector pXB7 has 10 unique cloning sites; pECAN8 contains 4 cloning sites within the lacZ gene permitting rapid detection of DNA inserts in the presence of Xgal. The third vector, pKBX, has a lower transformation frequency but adds kanamycin resistance as a selectable gene for shuttle vectors of cyanobacteria. |
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| Keywords: | Shuttle vector multiple cloning sites Cyanobacteria E. coli |
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