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Preliminary development of a polymerase chain reaction assay forAnaplasma marginale in ticks |
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| Authors: | R W Stich J A Bantle K M Kocan I S Eriks G H Palmer |
| Institution: | (1) Department of Veterinary Parasitology, Oklahoma State University, 74078 Stillwater, Oklahoma, USA;(2) Department of Microbiology and Public Health, Oklahoma State University, 74078 Stillwater, Oklahoma, USA;(3) Department of Zoology, Oklahoma State University, 74078 Stillwater, Oklahoma, USA;(4) Department of Veterinary Pathology, Oklahoma State University, 74078 Stillwater, Oklahoma, USA;(5) Department of Veterinary Microbiology and Pathology, Washington State University, 99164 Pullman, Washington, USA |
| Abstract: | Summary Polymerase chain reaction (PCR) was applied for detection ofAnaplasma marginale in tissues of maleDermacentor andersoni. Primer sequences were derived from the gene for the MSP1β surface protein ofA. marginale (Florida isolate). Optimum PCR conditions were used to detectA. marginale in individual bisected ticks and salivary glands; associated control tissues were negative. |
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