Purine mutants of mammalian cell lines. I. Accumulation of formylglycinamide ribotide by purine mutants of Chinese hamster ovary cells |
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Authors: | Rhoda I. Feldman Milton W. Taylor |
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Affiliation: | 1. Department of Microbiology, Indiana University, Bloomington, Indiana
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Abstract: | Mutants unable to perform de novo biosynthesis of purines have been isolated from cultures of mutagen-treated Chinese hamster ovary cells using bromodeoxyuridine selection techniques. Accumulation of C 14 -labeled formylglycinamide ribotide by suspension cultures of mutant cells incubated with glycine-C 14 suggested that the defect leading to auxotrophy most probably involves the gene coding for formylglycinamide amidotransferase, (E.C. 6.3, 5.3), the fourth enzyme in the de novo purine biosynthetic pathway. Direct assay of formylglycinamide amidotransferase activity in cell-free extracts prepared from mutant and parental cells has demonstrated the absence of amidotransferase activity in mutant derived extracts. |
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