Isolation and characterization of an adriamycin-resistant breast tumor cell line |
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Authors: | Sandra L Schneider Suzanne A W Fuqua Kermit V Speeg Atul K Tandon William L Mc Guire |
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Institution: | (1) Present address: Department of Medicine Divisions of Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284 San Antonio, Texas;(2) Gastronenterology/Nutrition, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284 San Antonio, Texas |
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Abstract: | Summary An adriamycin-resistant human breast tumor cell line MDA-A1
R
was generated by step-wise selection in increasing concentrations of drug from the parent cell line MDA-MB-231. MDA-A1
R
cells grow as loosely attached cell aggregates with a doubling time of 28–32 h; the MDA-MB-231 parent cell line grows as
a standard monolayer culture with a 20-h doubling time. The MDA-A1
R
cell line is highly resistant to adriamycin compared to the parent cell line, and is cross-resistant to velban and colchicine
suggestive of a multidrug resistance (MDR) phenotype. MDA-A1
R
cells exhibit reduced net adriamycin conent as compared to the parent cell line. The MDR-associated P-glycoprotein gene is
amplified approximately 10-to 30-fold in MDA-A1
R
cells. P-glycoprotein sequences are overexpressed in the resistant cells and are stable for up to 13 wk after drug removal.
Moreover, MDA-A1
R
cells show the presence of very high levels of P-glycoprotein. MDA-A1
R
is thus an in vitro model system to study the mechanism of MDR in human breast cancer.
This work was supported in part by grant C30195 from the National Institute of health, Bethesda, MD. Portion of this study
appeared as a poster presentation at the Tissue Culture Association meeting, Las Vegas, 1988. |
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Keywords: | adriamycin drug-resistance breast tumor cells p170 in vitro growth kinetics |
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