Double-gating mechanism and diversity of an adenosine triphosphate (ATP)-sensitive K+ channel in neurons acutely dissociated from rat neocortex |
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Authors: | Zhenqing Tong Xiangdong Tang and Wenjun Yang |
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Institution: | (1) Department of Physiology, First Military Medical University, 510515 Guangzhou, China;(2) Present address: Department of Neurology, Nanfang Hospital, 510515 Guangzhou, China |
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Abstract: | Classically, ion channels are classified into 2 groups: chemical-sensitive (ligand-gated) and voltage sensitive channels.
Single ATP-sensitive K+ (K-ATP) channel currents were recorded in acutely dissociated rat neocortical neurons using patch clamp technique. A type
of K-ATP channel has been found to be gated not only by intracellular ATP, but also by membrane potential (Vm), and proved to be a novel mechanism underlying the gating of ion channels, namely bi-gating mechanism. The results also
show that the K-ATP channels possess heterogeneity and diversity. These types of K-ATP channels have been identified in 40.12%
of all patches, which are different in activation-threshold and voltage-sensitivity. The present experiment studied the type-3
K-ATP channel with a unitary conductance of about 80 pS in detail (n = 15). Taking account of all the available data, a variety of K-ATP channels are suggested to exist in body, and one type
of them is bi-gated by both chemical substances and membrane potentials. This property of the K-ATP channels may be related
to their pathophysiological function.
Project supported by the National Natural Science Foundation of China and Natural Science Foundation of Guangdong Province |
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Keywords: | ATP-sensitive K+ channel gating kinetics patch clamp dissociated neuron rat |
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