Induction of B lymphocyte tolerance by an oligovalent antigen. I. Influence of the read-out system. |
| |
Authors: | J W Stocker G J Nossal |
| |
Institution: | The Walter and Eliza Hall Institute of Medical Research, Victoria 3050, Australia |
| |
Abstract: | A single intravenous injection of deaggregated preparations of lightly substituted dinitrophenylated human gamma globulin (DNP-HGG) induced DNP-specific tolerance in adult CBA mice, as judged by their failure to mount an IgM anti-DNP antibody-forming cell (AFC) response following challenge with the thymus-independent antigen, polymerized flagellin substituted with DNP (DNP-POL). Tolerance was also readily achieved in nude mice. Experiments using bovine serum albumin as the DNP carrier in both strains suggested that this was a less effective carrier for tolerance induction. The spleen cells from mice injected with DNP-HGG failed to respond to challenge with DNP-POL in vitro, but marked recovery of responsiveness occurred when the cells were challenged after adoptive transfer.These observations indicate that tolerance among antibody-forming cell precursors may selectively affect subpopulations. They further show that the choice of a read-out system used to analyze tolerance in B cells may critically influence the results. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|