Establishment of a resource population of SLA haplotype-defined Korean native pigs |
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Authors: | Han-Ok Cho Chak-Sum Ho Yu-Joo Lee In-Cheol Cho Sung-Soo Lee Moon-Suck Ko Chankyu Park Douglas M Smith Jin-Tae Jeon Jun-Heon Lee |
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Institution: | (1) Transplant Immunology Laboratory, Department of Pathology, Baylor University Medical Center, 3500 Gaston Avenue, Dallas, TX 75246, USA;(2) Institute of Biomedical Studies, Baylor University, Waco, TX 76798, USA;(3) Present address: Department of Medicine, University of Massachusetts School of Medicine, LRB, 370R, Worcester, MA 01655, USA;(4) Department of Animal Sciences, 382 Edward R. Madigan Laboratory, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA; |
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Abstract: | The highly polymorphic porcine major histocompatibility complex (MHC), or the swine leukocyte antigens (SLA), has been repeatedly
associated with variations in swine immune response to pathogens and vaccines as well as with production traits. The SLA antigens
are also important targets for immunological recognition of foreign tissue grafts. We recently established a resource population
of Korean native pigs as models for human transplantation and xenotransplantation research. In this study, 115 animals derived
from three generations of the Korean native pigs were genotyped for three SLA class I (SLA-2, SLA-3 and SLA-1) and three SLA
class II loci (DRB1, DQB1, DQA) using PCR with sequence-specific primers (PCR-SSP) at the allele group resolution. A total
of seven SLA haplotypes (Lr-5.34, Lr-7.23, Lr-31.13, Lr-56.23, Lr-56.30, Lr-59.1, Lr-65.34), comprising six unique class I
and five unique class II haplotypes, were characterized in the founding animals. Class I haplotype Lr-65.0 and class II haplotype
Lr-0.34 were novel; and together with Lr-56.0 these haplotypes appeared to be breed-specific. In the progeny population, Lr-7.23
and Lr-56.30 appeared to be the most prevalent haplotypes with frequencies of 34.7% and 31.6%, respectively; the overall homozygosity
was 27.4%. This resource population of SLA-defined Korean native pigs will be useful as large animal models for various transplantation
and xenotransplantation experiments, as well as for dissecting the roles of SLA proteins in swine disease resistance and production
traits. |
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