Optimization of nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20

We aimed to optimize a nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20. Xylanase production with lignocellulosic material was optimized in two steps using DeMeo’s fractional factorial design. A 3.4-fold increase in xylanase production (313.3 U/mL) was achieved using the optimized culture medium consisting of (g/L): K₂HPO₄, 2; MgSO₄·7H₂O, 0.3; CaCl₂·2H₂O, 0.01; NaCl, 2; peptone, 5 yeast extract, 4; and wheat bran, 50. _{B. pumilus} B20 produced a high level of xylanase, which may have potential industrial application.