CD40 expression in Wehi-164 cell line |
| |
| Authors: | Mohammad Hossein Karimi Padideh Ebadi Ali Akbar Pourfathollah Zahra Soheila Soheili Seyed Mohammad Moazzeni |
| |
| Affiliation: | (1) Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;(2) Faculty of Medicine, Islamic Azad University (IAU), Kazerun Branch, Kazerun, Iran;(3) Immunology Department, School of Medical Science, Tarbiat Modares University, Tehran, Iran;(4) Biochemistry Department, National Center of Genetic Engineering and Biotechnology, Tehran, Iran |
| |
| Abstract: | CD40-CD154 interaction is an important process for cellular and humoral immunity regulation and can be effective in the body’s defense against tumors. In the present study, we evaluated the expression of CD40 in Wehi-164 cell line. CD40 expressions on the cell surface and in the cytoplasm were assessed by flow cytometry and intracellular staining assay, respectively. Also, the mRNA expression was identified by real time-PCR. The obtained results showed the high mRNA and cytoplasmic protein expression of CD40 but no surface expression. These results suggest that the Wehi-164 cell line down regulates expression of CD40 on the surface for evasion of immune system. |
| |
| Keywords: | Wehi-164 CD40 Cytotoxicity |
| 本文献已被 SpringerLink 等数据库收录！ |
|
