Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein |
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| Authors: | Jaimie A Schnell Shuyou Han Brian L Miki Douglas A Johnson |
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| Institution: | (1) Ottawa-Carleton Institute of Biology, University of Ottawa, PO Box 450, Station A, Ottawa, ON, K1N 6N5, Canada;(2) Bioproducts and Bioprocesses Research Branch, Agriculture and Agri-Food Canada, Ottawa, ON, K1A OC6, Canada;(3) Present address: Plant and Biotechnology Risk Assessment Unit, Canadian Food Inspection Agency, 1400 Merivale Rd., Ottawa, ON, K1A 0Y9, Canada;(4) Present address: Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, ON, N5V 4T3, Canada; |
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| Abstract: | Elements that contribute to the high, stable yield of soybean peroxidase (SBP) in soybean seed coats can be exploited in the
development of this tissue as a protein production platform. SBP contains an N-terminal and a C-terminal propeptide that are
predicted to direct vacuolar targeting; this may be one factor that contributes to its high yield and stability. We characterized
the function of the SBP propeptides and investigated their ability to increase the yield of a foreign protein in a heterologous
plant system. SBP propeptides are functional signal peptides capable of directing vacuolar transport in Arabidopsis. The use of these propeptides as well as an endoplasmic reticulum (ER)-retention signal to direct a foreign protein to the
apoplast, ER, or vacuole can significantly increase yield and will therefore be useful for the development of the seed coat
as a protein production platform. We also demonstrate that growth conditions may have a significant impact on the yield of
a foreign protein and that this may be subcellular compartment-specific. |
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