Antioxidant effect of Ajuga iva aqueous extract in streptozotocin-induced diabetic rats |
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Authors: | D Taleb-Senouci H Ghomari D Krouf S Bouderbala J Prost MA Lacaille-Dubois M Bouchenak |
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Institution: | 1. Laboratoire de Nutrition Clinique et Métabolique, Département de Biologie, Faculté des Sciences, Université d’Oran Es Sénia, 31000 Oran, Algeria;2. UPRES Lipides & Nutrition, Faculté des Sciences Gabriel, Université de Bourgogne, 21000 Dijon, France;3. Laboratoire de Pharmacognosie, Faculté de Pharmacie, Unité de Molécules d’Intérêt Biologique, UMIB, UPRES EA 3660, Université de Bourgogne, 21079 Dijon, France |
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Abstract: | The purpose of this study was to investigate the possible antioxidant effect of an aqueous extract of Ajuga iva (Ai) in streptozotocin (STZ)-induced diabetic rats. Twelve diabetic rats were divided into two groups fed a casein diet supplemented or not with Ai (0.5%), for 4 weeks. In vitro, the Ai extract possessed a very high antioxidant effect (1 mg/ml was similar to those of trolox 300 mmol/l). The results indicated that plasma thiobarbituric acid reactive substances (TBARS) values were reduced by 41% in Ai-treated compared with untreated diabetic rats. TBARS concentrations were lower 1.5-fold in liver, 1.8-fold in heart, 1.9-fold in muscle and 2.1-fold in brain in Ai-treated than untreated group. In erythrocytes, Ai treatment increased significantly the activities of glutathione peroxidase (GSH-Px) (+25%) and glutathione reductase (GSSH-Red) (+22%). Superoxide dismutase activity was increased in muscle (+22%), while GSH-Px activity was significantly higher in liver (+28%), heart (+40%) and kidney (+45%) in Ai-treated compared with untreated group. Liver and muscle GSSH-Red activity was, respectively, 1.6- and 1.5-fold higher in Ai-treated than untreated diabetic group. Catalase activity was significantly increased in heart (+36%) and brain (+32%) in Ai-treated than untreated group. Ai treatment decreased plasma nitric oxide (?33%), carbonyls (?44%) and carotenoids (?68%) concentrations. In conclusion, this study indicates that Ajuga iva aqueous extract improves the antioxidant status by reducing lipid peroxidation and enhancing the antioxidant enzymes activities in plasma, erythrocytes and tissues of diabetic rats. |
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