Determination of captopril in plasma by high-performance liquid chromatography for pharmacokinetic studies |
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Authors: | C. Arroyo,C. Ló pez-Calull ,L. Garcí a-Capdevila,I. Gich,M. Barbanoj,J. Bonal |
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Affiliation: | C. Arroyo, C. López-Calull, L. García-Capdevila, I. Gich, M. Barbanoj,J. Bonal |
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Abstract: | A high-performance liquid chromatographic method is described for the determination of free captopril in human plasma. (NAC) was used as an internal standard. Plasma samples were immediately derivatized with N-(1-pyrenyl)maleimide (NPM) and stabilized with 11 M HCl. The drug of interest was isolated using a liquid-liquid extraction with ethyl acetate and separation was obtained using a reversed-phase column under isocratic conditions with fluorescence detection. The sample volume was 150 μl plasma. The intra- and inter-day accuracy and precision, determined as relative error and coefficient of variation respectively, were lessthan 10%. The lower limit of quantitation, based on standards with acceptable coefficients of variation, was 25 ng/ml. No endogenous compounds were found to interfere. The linearity was assessed in the range of 25–600 ng/ml. This method has been demonstrated to be suitable for pharmacokinetic studies in humans. |
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Keywords: | Captopril |
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