| 免标记光学纳米生物传感器的研究 |
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| 引用本文： | 卢金辉,张娟琨,赵晶,吴艳,李丽,王达飞. 免标记光学纳米生物传感器的研究[J]. 生物加工过程, 2009, 7(5): 73-76 |
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| 作者姓名： | 卢金辉 张娟琨 赵晶 吴艳 李丽 王达飞 |
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| 作者单位： | 天津科技大学,生物工程学院,天津市工业微生物重点实验室,天津,300457 |
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| 摘 要： | 利用发光稳定的多孔硅，当蛋白质溶液固定到多孔硅表面时，其发光强度和反射光谱的峰位会随着被测物质量浓度的变化而发生变化的特性。制备免标记光学纳米生物传感器来检测牛血清白蛋白（BSA），把不同质量浓度的BSA溶液固定到多孔硅上测量反射光谱的峰位和荧光强度的变化。实验结果表明：反射光谱的峰位与牛血清白蛋白的浓度呈线性关系：y=1．4189x＋366．31，R^2=0．9898；荧光强度的降低量与BSA溶液的浓度呈线性关系，检测限为8×10^-9 mol／L。
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| 关 键 词： | 免标记生物传感器 纳米多孔硅 反射光谱 |
Label-free optical nano-biosensors |
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| LU Jin-hui,ZHANG Juan-kun,ZHAO Jing,WU Yan,LI Li,WANG Da-fei. Label-free optical nano-biosensors[J]. Chinese Journal of Bioprocess Engineering, 2009, 7(5): 73-76 |
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| Authors: | LU Jin-hui ZHANG Juan-kun ZHAO Jing WU Yan LI Li WANG Da-fei |
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| Affiliation: | ( Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China) |
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| Abstract: | A stable light emittingporous silicon （PS）chip was fabricated. When the protein was deposited on the PS chip, the intensi.ty of peaks and the UV-visible spectra of the reflectance light changed with the solution concentration. A label-free sensor for detecting bovine serum albumin was assembled. The linear equation of the reflectance and the concentration was y = 1. 418 9x ＋366.31, R^2 =0. 9898. The detection limit was 8 ×10^-9 mol/L. |
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| Keywords: | label-free biosensor nano-porous silicon reflectance spectroseopy |
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