Structural Basis for the Biological Effects of Pr(III) Ions: Alteration of Cell Membrane Permeability |
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| Authors: | Liu Peng Zhang Weiying Li Xi Liu Yi |
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| Affiliation: | (1) School of Sciences, Wuhan University of Technology, Wuhan, 430070, People’s Republic of China;(2) College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People’s Republic of China;(3) Department of Applied Chemistry, School of Sciences, Wuhan University of Technology, Wuhan, 430070, People’s Republic of China |
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| Abstract: | The biological effects of rare-earth ions on the organism have been studied using Pr3+ as a probe ion and Escherichia coli cell as a target. Atomic force microscopy (AFM) observation of the surface of E. coli cells shows that the presence of Pr3+ substantially changes the structure of the outer membrane. By induced coupled plasma-mass spectrometry (ICP-MS), more Cu2+ was found in the cells grown in the presence of Pr3+, indicating changes of cell permeability. Using energy dispersive X-ray spectroscopy (EDX), Ca2+ is found on the outer surface of the original cell. It is proposed that Pr3+ can replace Ca2+ from the binding sites because of their close ionic radii and similar ligand speciality. |
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| Keywords: | Permeability Lipopolysaccharide (LPS) Pr3+ Membrane Surface |
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