Expression of human immunodeficiency virus genes using baculovirus expression system |
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Authors: | C Yong Kang |
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Institution: | (1) Faculty of Science, Western Science Centre, University of Western Ontario, N6A 5B7 London, Ontario, Canada |
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Abstract: | The structural protein genes of HIV-1 and HIV-2 have been expressed inSpodoptera frugiperda (SF) cells using baculovirus expression system. The noncoding flanking sequences of HIV structural genes were removed and
a putative ribosome binding site was placed in front of the open reading frame of each gene by using crossover linker mutagenesis.
The coding sequences of thegag, pol, env, andvif proteins were inserted intoAutographa californica nuclear polyhedrosis virus (AcNPV) so that HIV genes were under the control of the AcNPV polyhedrin promoter. All recombinant
AcNPV-infected SF cells express high levels of HIV structural proteins. Detailed strategies of recombinant AcNPV construction
for high level protein expression are presented. |
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Keywords: | Human immunodeficiency virus viral proteins AIDs gene expression baculovirus expression system vaccines |
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