Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria |
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| Authors: | T J Hefley N S Krieger P H Stern |
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| Affiliation: | 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi''an, Shaanxi 710069, China;2. College of chemistry & materials science, Northwest University, Taibai North Road 229, Xi''an, Shaanxi 710069, China;3. The High School Attached to Northwest University, Xi''an 710069, China;4. Microbiology institute of Shannxi, Xi''an 710043, China |
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| Abstract: | A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resorption has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide. |
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