Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant,antibacterial, hemolytic and in vivo cutaneous wound healing effects |
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| Authors: | Sawssen Hajji Sameh Ben Khedir Ibtissem Hamza-Mnif Marwa Hamdi Ines Jedidi Rim Kallel Sami Boufi Moncef Nasri |
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| Affiliation: | 1. Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173, 3038 Sfax, Tunisia;2. Laboratory of Histology and Embryology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia;3. Laboratory of Hematology, CHU Habib Bourguiba, University Hospital, Sfax, Tunisia;4. Laboratory of Pathological Anatomy and Cytology, CHU Habib Bourguiba, University Hospital, Sfax, Tunisia;5. LMSE, University of Sfax, Faculty of Science of Sfax, Tunisia |
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| Abstract: | The aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190–200?nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the results revealed that they exhibited higher antioxidant activity than CS powder. Moreover, CS-AgNPs were characterized by a low cytotoxicity effect at 5–200?μg/ml against Chinese Hamster Ovary (CHO-K1) cells. In addition, the prepared CS-Ag NPs were found to promote significantly the wound healing, as determined by the wound contraction ratio and histological examination. A significant improvement in wound healing progression and in oxidative stress damage were observed for CS, CS-PVA and CS-AgNPs-treated wound tissues, when compared to control and CICAFLORA®-treated groups. The wound healing effect could be attributed to the antibacterial and antioxidant synergy of AgNPs and CS. Results strongly support the possibility of using CS-AgNPs for wound care applications. |
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| Keywords: | Chitosan-silver nanoparticles Structural characterization Antioxidant and antibacterial activities Cytotoxicity effect Wound-healing efficiency |
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