| 黑曲霉AF-98固体发酵产纤维素酶的产酶条件研究 |
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| 引用本文： | 伍红,陆兆新,吕玫,徐源.黑曲霉AF-98固体发酵产纤维素酶的产酶条件研究[J].菌物学报,2006,25(3):475-480. |
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| 作者姓名： | 伍红 陆兆新 吕玫 徐源 |
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| 作者单位： | 1. 西南民族大学生命科学与技术学院,成都,610041
2. 南京农业大学食品科技学院,南京,210095 |
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| 摘 要： | 通过单因子及正交试验,对黑曲霉AF-98固体发酵产纤维素酶的产酶条件进行了探讨。其优化的产酶条件为:甘蔗渣3g,麸皮2g,加含尿素为0.15%的Mandels营养液25mL(加水比1:5),调初始pH5.0,28℃发酵72h。在此优化条件下,纤维素酶活力可达7.56u/g干曲。
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| 关 键 词： | 真菌 单因子试验 培养条件优化 麸皮 |
| 文章编号： | 1672-6472（2006）03-0475-0480 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-112006-04-03 |
Production of cellulase by Aspergillus niger AF-98 in solid-state cultivation |
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| Authors: | WU Hong LU Zhao-Xin LU Mei XU Yuan |
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| Institution: | 1Life Sciences and Technical College of Southwest National University, Chengdu 610041, 2Food Science and Technology Department, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | The culture conditions for the production of extra-cellular cellulase in solid-state fermentation by Aspergillus niger AF-98 were studied through single factor experiments and orthogonal test. The results showed that the optimal culture conditions were as follows: medium prepared from 2g bagasse, 3g wheat bran and 25mL Mandels nutritional liquid containing 0.15% carbamide , adjustment of initial pH to 5.0, at 28℃ for 72h. Under these optimal fermentation conditions, cellulase activity reached 7.56IU/g dry medium. |
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| Keywords: | Eumycete Simple factor experiment Optimization of culture condition Wheat bran |
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