Conditions affecting cell surface properties of human intestinal bifidobacteria |
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Authors: | Enrica Canzi Simone Guglielmetti Diego Mora Isabella Tamagnini Carlo Parini |
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Institution: | (1) Department of Food Science and Microbiology, Agricultural, Food, Ecological Microbiology Section, University of Milan, Via Celoria 2, 20133 Milan, Italy;(2) Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milan, Via Celoria 2, 20133 Milan, Italy |
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Abstract: | The cell surface properties of human intestinal bifidobacteria have been characterized for 30 strains isolated from a fecal
sample. Strain identification to the species level was obtained by restriction analysis of the amplified 16S rRNA gene and
confirmed by DNA/DNA reassociation experiments. The isolates were grouped in four genetically homogeneous clusters whose members
belonged to Bifidobacterium bifidum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium pseudocatenulatum species. Cell surface properties of Bifidobacterium strains were evaluated by determining the level of hydrophobicity, adhesion to hydrocarbons and contact angle measurements,
and their autoaggregation ability. The results showed high and homogeneous level of hydrophobicity in all tested strains when
contact angle measurements values were considered. On the contrary, autoaggregation assays and bacterial adhesion to hydrocarbons
detected interesting differences in cell surface properties among the tested Bifidobacterium strains. The highest levels of autoaggregation, detected in B. bifidum and B. adolescentis strains, were strictly dependent on the pH of the medium. Moreover, protease treatment experiments suggested that proteins
had a key role in the autoaggregating ability of B. bifidum and B. adolescentis strains. |
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Keywords: | Autoaggregation Bifidobacterium species Genetic polymorphism Hydrophobicity Probiotics |
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