Use of purified antipeptide sera of selected specificity for the detection of the simian virus 40 large T antigen by western blotting |
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Authors: | Jean Bernard Dietrich |
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Affiliation: | (1) Institut für Immunobiologie der Albert-Ludwigs-Universität, Stefan Meier Strasse 8, Freiburg i. Br., West Germany;(2) Present address: Institut de Biologie Moléculaire et Cellulaire du CNRS, 15 rue Descartes, 67084 STRASBOURG Cedex, France |
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Abstract: | Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [15S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins were transferred electrophoretically on nitrocellulose paper. T antigen was detected on nitrocellulose strips by using for the first time, specific, purified antipeptide monoclonal antibodies directed against the N- and C-terminal portions of the molecule, and125I-labeled Protein A. |
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