与抑制K562细胞向红系分化相关的Attractin基因的分离和初步鉴定

应用差异显示PCR（DDRT-PCR）方法分离野生型K562细胞株和能表达人β－珠蛋白基因的变种K562细胞株的4个差异cDNA片段，序列测定后，挑选差异最明显的片段dd1，经过RT-PCR、Northern 印迹鉴定确实为两株细胞间的差异片段，测序后经同源性分析等，发现其所对应的基因是吸引素（Attractin,GenBank注册号AF106861）。 根据其结构预测，Attractin有可能是K562 细胞表面一个黏附因子受体，与抑制K562细胞向红系分化有关。 Abstract:Differential display PCR method was used to isolate four differential display fragments between the wild type K562 cell line and the variant type K562 cell line that expressed the human β-globin gene.After sequencing,the most remarkable different fragment,named dd1,was selected for further study.The analysis of RT-PCR and Northern blot hybridization showed that dd1 was exactly the differentiation fragment between the two cell lines.The homology analysis indicated that dd1 was matched to Attractin (GeneBank registration No.AF106861).It might be an adhesion receptor related to inhibiting erythroid differentiation based on its structure.

Isolation and Identification of a Gene:Attractin Related to Inhibiting Erythroid Differentiation in K562 Cells

Differential display PCR method was used to isolate four differential display fragments between the wild type K562 cell line and the variant type K562 cell line that expressed the human beta-globin gene. After sequencing, the most remarkable different fragment,named dd1,was selected for further study. The analysis of RT-PCR and Northern blot hybridization showed that dd1 was exactly the differentiation fragment between the two cell lines. The homology analysis indicated that dd1 was matched to Attractin (GeneBank registration No.AF106861). It might be an adhesion receptor related to inhibiting erythroid differentiation based on its structure.