Tumor necrosis factor alpha induction in human monocytes |
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| Authors: | N I Misuno O A Osipovich A B Sudarikov G L Idelson T S Kolesnikova A V Panyutich N N Voitenok |
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| Institution: | Byelorussian Institute of Hematology and Blood Transfusion, Minsk, USSR. |
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| Abstract: | The present study was undertaken to assess the presence of tumor necrosis factor (TNF)-alpha mRNA and protein in circulating human blood monocytes and to study the TNF-alpha gene expression in human monocytes isolated by continuous Percoll gradient fractionation. The technique of RNA isolation directly from the blood samples was used to study TNF-alpha mRNA expression in circulating human blood leukocytes. It was shown that human blood leukocytes of healthy donors contained no presynthesized pool of TNF-alpha mRNA as well as no TNF-alpha protein. It was found that early pretreatment with cycloheximide interferes with TNF-alpha mRNA induction by Staphylococcus aureus. |
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