Purification and characterization of genetically polymorphic deoxyribonuclease I from human kidney. |
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| Authors: | D Nadano T Yasuda K Kishi |
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| Institution: | Department of Legal Medicine, Fukui Medical School. |
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| Abstract: | Deoxyribonuclease I (DNase I) was purified about 850,000-fold from human kidney using a rabbit anti-human urine DNase I antibody and sensitive DNase I activity assay. On SDS-PAGE, the purified kidney DNase I gave a single major band, and its molecular mass was estimated to be 38,000 Da. The activity of purified kidney DNase I was dependent on the presence of Mg2+ and Ca2+. G-Actin inhibited the activity, as did the anti-urine DNase I antibody. The properties of the kidney DNase I were the same as those of urine DNase I. |
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