Separation of precultured pollen from Nicotiana tabacum by aqueous polymer two-phase partition |
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Authors: | Boel K Mårtensson Susanne Widell |
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Institution: | (1) Department of Plant Physiology, University of Lund, Box 7007, S-220 07 Lund, Sweden |
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Abstract: | Pollen isolated from cold treated and precultivated anthers of tobacco (Nicotiana tabacum L. var. Wisconsin 38) were separated into different fractions with counter-current distribution using an aqueous Dextran-polyethylene glycol two-phase system. It was possible to distinguish among eight pollen classes differing in developmental stage and in partitioning. A part of each fraction was cultivated for analysis of embryo formation. This was highest in a fraction with an intermediate to high partition in the phase system. Enriched in this fraction were also pollen that were fairly well stained with acetocarmine, contained several nuclei and had a relatively low mitochondrial activity. The enrichment of embryogenic pollen offers several advantages especially to physiological studies on embryogenesis. |
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Keywords: | embryogenesis fractionation haploid pollen culture |
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